Skip to main content
. 2022 May 20;298(7):102059. doi: 10.1016/j.jbc.2022.102059

Figure 3.

Figure 3

Ectopic RORα expression suppresses the TGF-β-induced EMT.A, RORα expression was verified by Western blot after control, and RORα expression vectors were introduced in MCF10A cells. B, phase contrast images of 10 ng/ml TGF-β-treated control and RORα-expressing MCF10A cells in 2D culture. Bar represents 20 μm. C, expression of epithelial and mesenchymal marker proteins was examined in 10 ng/ml TGF-β-treated control and RORα-expressing MCF10A cells. D, immunofluorescent images showing E-cadherin in the 10 ng/ml TGF-β-treated control and RORα-overexpressing MCF10A cells. Bar represents 10 μm. EMT, epithelial–mesenchymal transition; RORα, retinoid orphan nuclear receptor alpha; TGF-β, transforming growth factor-β.