Figure 7.

6h induces apoptosis in A549 and H460 cells.A, A549 and H460 cells were treated with 6h (2 IC₅₀) or colchicine (2 IC₅₀) for 24, 48, or 72 h, and then the nuclei were stained with Hoechst 33258, and photographed by Olympus inverted fluorescence microscope. The scale bar represents 50 μm. B, the apoptosis of A549 and H460 cells treated with 6h (2 IC₅₀) for 12, 24, 36, or 48 h was measured by flow cytometry with annexin V–FITC staining. The percentage of apoptosis rate in A549 and H460 cells was quantified. Changes of the expression of cell apoptosis–related proteins in A549 cells (C) or H460 cells (D) treated with 6h (2 IC₅₀) for indicated time (0, 12, 24, 36, or 48 h) were measured. The histogram in each panel indicated the relative band intensity generated form densitometric scans of three independent experiments on arbitrary densitometric units. ∗p < 0.05, ∗∗p < 0.01 versus control.