FIGURE 1.

Antibodies anti-Mce1a are biomarkers for the diagnosis and monitoring of HD. IgA (A), IgM (B), and IgG (C) antibody indices against Mce1A protein and PGL-I (D) in different groups of plasma samples tested. Statistical significance was determined by the Kruskal–Wallis test followed by the Dunn test. Data are presented as the median and interquartile range (IQR); significance was considered at p < 0.05, p < 0.01, p < 0.001, or p < 0.0001 as represented by *, **, ***, and ****, respectively. EC endemic controls (n = 20); HHC household contacts of HD patients in Parnaíba (n = 17); PAR-NC new cases of HD in Parnaíba (n = 26); CAR-TP patients treated in Colony Carpina (n = 19). The respective index was calculated by dividing the optical density (O. D 450 nm) of each sample by the cut-off, and indices above 1.0 were considered positive represented by horizontal dotted line.