TABLE 5.

Results of the artificial contamination tests; bold: critical value and one log level below.

	0 H enrichment
	Contamination level (CFU/10 g matrix)	CFU/g template (absolute)	LAMP groEL	LAMP nheB	qPCR groEL	qPCR nheB	Cultural
DSM31, DSM 4384,	0	0	0/12	0/12	0/12	0/12	0/12
MHI 3099, MHI 252	0–1	0.03 (alte) l	0/12	0/12	0/12	0/12	0/12
	1–10	0.33 (alte) l	0/12	0/12	0/12	0/12	1/12
	10–100	3.27 (alte)	0/12	0/12	0/12	0/12	1/12
	100–1,000	32.7 alte	0/12	0/12	0/12	0/12	5/12
	1,000–10,000	327 0 alte	1/12	0/12	0/12	2/12	10/12
	10,000–100,000	3.27 × 103 02005 × 103	3/12	1/12	2/12	2/12	12/12
	100,000–1,000,000	3.27×104 020,5 × 104	7/12	2/12	1/12	8/12	12/12
	1,000,000–10,000,000	3.27×105 02005×105	10/12	6/12	4/12	12/12	12/12
	6 H enrichment
	Contamination level (CFU/10 g matrix)	CFU/g template (absolute)	LAMP groEL	LAMP nheB	qPCR groEL	qPCR nheB	Cultural
DSM31, DSM 4384,	0	0	0/12	0/12	0/12	0/12	0/12
MHI 3099, MHI 252	0–1	0.03 (alte) l	0/12	0/12	0/12	0/12	0/12
	1–10	0.33 (alte) l	2/12	1/12	0/12	1/12	4/12
	10–100	3.27 (alte)	6/12	3/12	2/12	4/12	7/12
	100–1,000	32.7 alte	9/12	3/12	3/12	8/12	12/12
	1,000–10,000	327 0 alte	9/12	6/12	4/12	10/12	11/12
	10,000–100,000	3.27 × 103 02005 × 103	9/12	8/12	7/12	12/12	12/12
	100,000–1,000,000	3.27×104 020,5×104	10/12	9/12	8/12	12/12	12/12
	1,000,000–10,000,000	3.27×105 02005×105	12/12	11/12	9/12	12/12	12/12

100–90% 80–50%  

90–80% <50% Negative