TABLE 6.
Results of native sample analysis (n = 100).
Contamination level (CFU/g), 0 h | Cultural positive | groEL LAMP | groEL qPCR | nheB LAMP | nheB qPCR | MALDI + |
>100–101 | 12 | 1 | 1 | 0 | 2 | 12 |
101–102 | 1 | 0 | 0 | 0 | 0 | 1 |
102–103 | 1 | 1 | 1 | 0 | 0 | 1 |
103–104 | 2 | 2 | 2 | 0 | 1 | 2 |
104–105 | 0 | 0 | 0 | 0 | 0 | 0 |
105–106 | 1 | 1 | 0 | 0 | 0 | 1 |
Total positive: | 17 | 5 | 4 | 0 | 3 | 17* |
Add. false positive: | – | 0 | 0 | 0 | 2 | |
| ||||||
Contamination level (CFU/g), 6 h | Cultural positive | groEL LAMP | groEL qPCR | nheB LAMP | nheB qPCR | MALDI + |
| ||||||
>100–101 | 6 | 0 | 2 | 0 | 1 | 6 |
101–102 | 0 | 0 | 0 | 0 | 0 | 0 |
102–103 | 8 | 2 | 2 | 0 | 1 | 8 |
103–104 | 20 | 5 | 6 | 2 | 6 | 20 |
104–105 | 10 | 7 | 5 | 1 | 5 | 10 |
105–106 | 2 | 2 | 1 | 2 | 1 | 2 |
Total positive: | 46 | 16 | 16 | 5 | 14 | 46** |
Add. false positive: | – | 0 | 1 | 0 | 5 |
*1 isolate of B. thuringiensis, **2 isolates of B. thuringiensis, gray, critical value (from 105 CFU/g food).