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. 2022 Jun 11;15:100319. doi: 10.1016/j.mtbio.2022.100319

Fig. 3.

Fig. 3

IONP-Exos significantly promoted the proliferation, migration, and fibrogenesis of NIH3T3 fibroblasts. (A) IONP-Exos significantly promoted the proliferation of NIH3T3 fibroblasts, as demonstrated by the Cell Counting Kit-8 (CCK-8) assay. (B) Schematic illustration of the different parts of the transwell migration assay system. (C) IONP-Exos significantly promoted the migration of NIH3T3 fibroblasts as determined by the transwell assay after 24 ​h. (D) Quantitative analysis of the transwell assay. (E) IONP-Exos significantly promoted the migration ability of NIH3T3 fibroblasts as determined by the wound healing assay. (F) Quantitative analysis of the wound closure rates. (G–I) NIH3T3 fibroblasts were treated with IONP-Exos, BMSC-Exos, or PBS as the negative control, and the mRNA levels of Col I, Col III, and α-SMA were evaluated by qRT-PCR. (J) Western blot analysis of the expression of fibrotic-related proteins, including Col I, Col III, and α-SMA, GAPDH was used as a loading control. IONP: iron oxide nanoparticle; CCK-8: Cell Counting Kit-8; BMSC: bone mesenchymal stem cell. Each assay was performed in triplicate and/or carried out in at least three independent experiments; representative results are shown. ∗P ​< ​0.05; ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001. Scale bar: 100 ​μm.