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. 2022 Jun 10;15:100320. doi: 10.1016/j.mtbio.2022.100320

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 3 — Antioxidant efficiency of scaffolds and transformation of macrophage phenotype from M1 to M2. (A) DPPH scavenging capabilities by the CHS, CHS-PDA-2, CHS-PDA-1 and CHS-PDA-0.5 and (B) the images of DPPH scavenging of all groups for 1 h and 12 h. (C) Intracellular ROS-scavenging performance of CHS, CHS-PDA-2, CHS-PDA-1 and CHS-PDA-0.5. Scale bars, 100 μm. (D) Flowcytometry of DCFH-DA labeled Raw264.7 cells in fluorescein isothiocyanate FITC-A channel on the different scaffolds. The percentage of both sides were also shown. (E–G) The expression of anti-inflammatory factors CD206, Arg-1 and pro-inflammatory factors IL-6 and iNOS in macrophage (Raw264.7) after treated with different scaffolds by RT-PCR. Data represented mean ± SD (n = 3). ∗∗∗P ＜ 0.001, ∗∗P ＜ 0.01, ∗P ＜ 0.05, ns, P ＞ 0.05 compared with the LPS-only treated group.