Fig. 3.

UM with focally positive PRAME expression (case 16). a Cross-section of enucleation shows choroidal melanoma tumor toward the inferior pole (H&E stain). b Low-power image of the malignant melanoma cells in the tumor (H&E stain). c MART-1 immunolabeling (red cytosolic staining) highlights the tumor cells. d–f Focally positive PRAME immunolabeling with intermixed PRAME-positive (brown nuclear staining) and PRAME-negative tumor cells (no brown nuclear staining) present in the same enucleation. Nuclei in (b–f) have been counterstained with hematoxylin (blue). Scale bars, 2 mm (a), 50 μm (b–d), and 10 μm (e, f).