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. 2022 Jun 9;9:778941. doi: 10.3389/fcell.2021.778941

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Copyright © 2022 Zhang, Li, Li, Yang, Liao, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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METTL3 was a direct target of miR-373. (A,B) The expression of METTL3 mRNA and protein in normal and OA samples was determined by qRT-PCR and Western blotting. GAPDH was used as the endogenous reference for normalization. (C,D) The effect of miR-373 mimic or inhibitor on the expression of METTL3 mRNA and protein in cultured human chondrocytes was determined by qPCR and Western blotting. GAPDH was used as the endogenous reference for normalization. (E) The targeting specificity of miR-373 to METTL3 was verified by dual-luciferase reporter assay. A miR-373-resistant METTL3 was generated as the shown sequence. Luciferase activity based on either METTL3-WT or METTL3-MUT was normalized to the corresponding mimic NC control separately. p < 0.05 was marked as *, p < 0.01 was marked as **.