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. 2022 Jun 9;9:778941. doi: 10.3389/fcell.2021.778941

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Copyright © 2022 Zhang, Li, Li, Yang, Liao, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of METTL3 overturned the CREB-promoted autophagic flux. (A) The expression of METTL3 (left) and TFEB (right) were confirmed by qRT-PCR. GAPDH was used as an endogenous reference. (B) The changes of the protein of METTL3 and TFEB in the experiment above were determined by Western blotting. Quantified intensity of the bands was normalized to GAPDH to generate the column chart. (C) The changes of m6A of TFEB mRNA in different treatments above. (D) The impact on the autophagic flux was determined by the immuno-staining of LC3B. LC3B fluorescence intensity was measured and data from all groups were normalized to the TBHP group. (E) The changes of the essential regulators or markers of apoptosis and autolysosome were determined by Western blotting. Quantified intensity of the bands was normalized to GAPDH to generate the column chart. p < 0.05 was marked as *, p < 0.01 was marked as **, p < 0.001 was marked as ***.