Table 1.
The effect of different fermentation medium compounds on the production of yellow Monascus pigments in submerged fermentation for 10 days at 30°C and agitated at 150 rpm in the dark.
Composition | Compound | Concentration (g/L) | inMPs (U/ml) | exMPs (U/ml) | T-MPs (U/ml) | exMPs/T-MPs (%) | Increased by (%) |
---|---|---|---|---|---|---|---|
CK | – | – | 10.67 | 22.68 | 33.35 | 68.01 | – |
Growth factor | B1 | 0.2 | 17.79 | 29.82 | 47.61 | 62.63 | 42.76 |
B3 | 0.2 | 15.06 | 27.86 | 42.52 | 65.52 | 27.50 | |
B5 | 0.2 | 15.10 | 36.53 | 51.63 | 70.75 | 54.81 | |
B6 | 0.2 | 12.04 | 28.22 | 40.26 | 70.09 | 20.72 | |
VC | 0.2 | 14.57 | 32.28 | 46.85 | 68.90 | 40.48 | |
B5 | 0.1 | 10.76 | 24.84 | 35.60 | 69.78 | 6.75 | |
0.2 | 15.10 | 36.53 | 51.63 | 70.75 | 54.81 | ||
0.3 | 15.28 | 40.17 | 55.45 | 72.45 | 66.27 | ||
0.4 | 19.09 | 44.49 | 63.58 | 69.98 | 90.64 | ||
0.5 | 15.38 | 33.24 | 48.62 | 68.37 | 45.79 | ||
Nitrogen source | Peptone | 5 | 23.66 | 36.70 | 60.36 | 60.80 | 80.99 |
10 | 26.80 | 46.50 | 73.30 | 63.44 | 119.79 | ||
15 | 36.76 | 52.80 | 89.56 | 58.95 | 168.55 | ||
20 | 21.48 | 46.35 | 67.83 | 68.33 | 103.39 | ||
Malt extract | 5 | 27.64 | 54.65 | 82.29 | 66.41 | 146.75 | |
10 | 35.80 | 59.35 | 95.15 | 62.37 | 185.31 | ||
15 | 26.46 | 48.45 | 74.91 | 64.68 | 124.62 | ||
20 | 23.84 | 44.55 | 68.39 | 65.14 | 105.07 | ||
NaNO3 | 1 | 14.45 | 28.20 | 42.65 | 51.24 | 27.89 | |
3 | 19.90 | 45.65 | 65.55 | 69.64 | 96.55 | ||
5 | 28.40 | 51.50 | 79.90 | 64.46 | 139.58 | ||
7 | 18.59 | 39.46 | 58.05 | 67.96 | 74.06 | ||
9 | 11.85 | 32.80 | 44.65 | 73.46 | 33.88 | ||
Metal ion |
Mg2+ | 0.05 | 15.62 | 29.30 | 44.92 | 65.23 | 34.69 |
0.1 | 15.58 | 36.73 | 52.31 | 70.22 | 56.85 | ||
0.2 | 17.65 | 43.27 | 60.92 | 71.03 | 82.67 | ||
0.5 | 19.59 | 36.81 | 56.40 | 65.26 | 69.12 | ||
Mn2+ | 0.01 | 13.65 | 39.12 | 52.77 | 74.13 | 58.23 | |
0.05 | 15.17 | 48.16 | 63.33 | 76.05 | 89.90 | ||
0.1 | 19.63 | 54.88 | 74.51 | 73.65 | 123.42 | ||
0.2 | 17.46 | 34.08 | 51.54 | 66.12 | 54.54 | ||
Fe2+ | 0.5 | 12.35 | 29.57 | 41.92 | 70.55 | 25.70 | |
1 | 13.20 | 34.15 | 47.35 | 72.13 | 41.98 | ||
1.5 | 13.08 | 39.76 | 52.84 | 75.25 | 58.44 | ||
2 | 16.32 | 28.04 | 44.36 | 63.22 | 33.01 | ||
Ca2+ | 0.5 | 9.73 | 24.23 | 33.96 | 71.35 | 1.83 | |
1 | 13.01 | 30.91 | 43.92 | 70.38 | 31.69 | ||
1.5 | 17.16 | 36.92 | 54.08 | 68.26 | 62.16 | ||
2 | 11.60 | 25.30 | 36.90 | 68.56 | 10.64 | ||
Zn2+ | 0.05 | 17.99 | 47.03 | 65.02 | 72.33 | 94.96 | |
0.1 | 22.41 | 52.65 | 75.06 | 70.15 | 125.07 | ||
0.2 | 26.75 | 57.83 | 84.58 | 68.37 | 153.61 | ||
0.5 | 18.78 | 42.22 | 61.00 | 69.22 | 82.91 |
The data were expressed as the mean from three experiments.