Table 2.

Box–Behnken design for process parameters of yellow monascus pigments production by M. purpureus H14 in submerged fermentation for 10 days.

Compounds	Symbol	Coded levels
		−1		0	1
ZnSO4•7H2O	A	0.1		0.3	0.5
MnSO4•H2O	B	0.05		0.13	0.2
Malt extract	C	5		10	15
Vitamin B5	D	0.3		0.4	0.5
Run order	A	B	C	D	YMPs (U/ml)
					Experimental	Predicted
1	0	1	0	1	82.06	81.34
2	−1	0	1	0	98.56	98.76
3	−1	0	0	1	77.92	76.20
4	0	0	0	0	103.28	104.69
5	0	0	−1	1	77.52	76.76
6	−1	0	−1	0	75.26	77.41
7	1	0	1	0	91.66	90.82
8	0	0	0	0	105.88	104.69
9	0	−1	1	0	97.18	96.90
10	1	−1	0	0	93.67	91.89
11	0	1	−1	0	74.38	73.24
12	0	0	1	−1	91.48	92.65
13	0	1	1	0	95.71	94.23
14	0	−1	0	−1	75.37	74.30
15	0	−1	0	1	79.38	81.77
16	−1	−1	0	−1	82.94	81.13
17	1	0	0	−1	73.98	73.98
`18	0	0	0	0	104.56	104.69
19	1	1	0	0	68.94	71.17
20	−1	0	0	−1	89.16	88.15
21	0	−1	0	−1	87.04	89.07
22	0	0	−1	−1	80.24	79.12
23	1	0	−1	0	79.54	80.65
24	0	0	0	0	104.37	104.69
25	−1	1	0	0	84.45	86.64
26	1	0	0	1	86.38	85.66
27	0	−1	−1	0	86.32	86.07
28	0	0	0	0	105.36	104.69
29	0	0	1	1	93.21	94.75

The data were expressed as the mean from three experiments.