Table 3.

ANOVA for the effect of ZnSO₄•7H₂O, MnSO₄•H₂O, vitamin B5, and malt extract on yellow monascus pigment production and regression coefficients.

Source	Sum of squares-type III	df	Mean square	F value	p value Prob > F	Significance
Model	3249.06	14	232.08	62.12	<0.0001	**
A (ZnSO4•7H2O)	16.61	1	16.61	4.45	0.0535	–
B(MnSO4•H2O)	173.43	1	173.43	46.42	<0.0001	**
C (malt extract)	744.82	1	744.82	199.36	<0.0001	**
D (vitamin B5)	0.053	1	0.053	0.014	0.9066	–
AB	172.13	1	172.13	46.07	<0.0001	**
AC	31.25	1	31.25	8.36	0.0118	*
AD	139.71	1	139.71	37.40	<0.0001	**
BC	27.41	1	27.41	7.34	0.0170	*
BD	51.48	1	51.48	13.78	0.0023	*
CD	4.95	1	4.95	1.33	0.2690	–
A 2	828.56	1	828.56	221.77	<0.0001	**
B 2	739.81	1	739.81	198.02	<0.0001	**
C 2	272.13	1	272.13	72.84	<0.0001	**
D 2	996.09	1	996.09	266.61	<0.0001	**
Residual	52.31	14	3.74
Lack of fit	48.33	10	4.83	4.87	0.0704	–
Pure error	3.97	4	0.99
Corrected total	3301.36	28
SD	1.93
Mean	87.79
CV%	2.20
R 2	0.9842
R 2 adj	0.9683
R 2 pred	0.9138
Adep. pre	24.113

CV, coefficient of variation; R², coefficient of determination; R²_adj, adjusted R²; R²_pred, predicted R²; and Adep. Pre, adequate precision.

^**p < 0.01 highly significant.

^*p < 0.05, significant; −, not significant.