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. 2022 Jun 9;13:897376. doi: 10.3389/fpls.2022.897376

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Copyright © 2022 Liu, Ma, Wang, Chen, Ma and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression pattern of miR395c, PagATPS, and PagSULTR2;1 during vascular development in poplar. (A) qPCR analysis showing that the expression of mature miR395c in the apex and secondary xylem was higher than in the procambium, vascular cambium, and secondary phloem. (B) qPCR analysis showing that the expression of PagAPS1, PagAPS3/4, and PagSULTR2;1 in the cambium and secondary phloem was higher than in the secondary xylem. U6 and UBQ or actin was used as internal reference genes in miRNA qPCR and mRNA qPCR analysis, respectively. Error bars represent the standard error of three independent biological replicates. PC, procambium; VC, vascular cambium; SP, secondary phloem; SX, secondary xylem.