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. 2022 Jun 9;13:897376. doi: 10.3389/fpls.2022.897376

FIGURE 3.

FIGURE 3

Cross-sections of 2nd, 4th, and 8th internodes in 3-month-old wild-type and transgenic 84K poplars. (a) Cross-section of the 2nd internode in wild type. (b) Cross-section of the 2nd internode in miR395c-OE poplar. (c) Cross-section of the 4th internode in wild type. (d) Cross-section of the 4th internode in miR395c-OE poplar. (e) Cross-section of the 8th internode in the wild type. (f) Cross-section of the 8th internode in miR395c-OE poplar. pp, primary phloem; px, primary xylem; c, cambium; vc, vascular cambium; sp, secondary phloem; sx, secondary xylem. (g) Average area of vessels increased in transgenic plants. (h) Separation and safranin staining of secondary xylem cells in wild-type poplar. (i) Separation and safranin staining of secondary xylem cells in miR395c-OE poplar. Bar, 100 μm. xf, xylem fiber; ve, vessel. (j) Xylem vessel length increased in miR395c-OE poplar. (k) Xylem vessel width increased in miR395c-OE poplar. (l) Xylem fiber length increased in miR395c-OE poplar. (m) Xylem fiber width changed slightly in miR395c-OE poplar. Asterisks indicate significant difference between transgenic and WT plants using the Student’s t-test, ***p < 0.001.