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. 2022 Jun 10;26(2):253. doi: 10.3892/mmr.2022.12769

Table II.

Particle size and ζ-potential of DOTAP-based cationic liposomes and small interfering RNA lipoplexes.

Liposome Lipoplexb


Liposome Sizea, nm PDI ζ-potentiala, mV Sizea, nm PDI ζ-potentiala, mV
LP-DOTAP/DSPE 111.8±2.0 0.23±0.01 50.3±0.5 216.3±27.3 0.19±0.07 48.7±2.7
LP-DOTAP/DPPE 116.0±0.9 0.20±0.01 48.3±0.7 163.7±6.5 0.21±0.01 41.1±0.7
LP-DOTAP/DMPE 113.7±1.8 0.28±0.01 52.4±2.2 184.0±5.6 0.20±0.01 47.8±2.2
LP-DOTAP/DOPE 109.2±1.0 0.21±0.01 53.7±1.6 179.1±5.7 0.16±0.01 42.8±1.5
LP-DOTAP/POPE 111.7±1.4 0.24±0.02 49.6±1.0 175.8±6.5 0.21±0.01 40.3±0.4
LP-DOTAP/DSPC 93.3±0.8 0.22±0.01 48.9±1.4 228.7±1.5 0.26±0.00 32.2±0.7
LP-DOTAP/DPPC 112.8±0.8 0.26±0.00 46.7±0.7 200.8±2.8 0.23±0.00 26.6±2.8
LP-DOTAP/DMPC 107.2±1.0 0.25±0.00 46.3±2.7 240.5±1.9 0.28±0.02 31.3±1.1
LP-DOTAP/DOPC 102.2±0.9 0.24±0.01 48.6±3.0 227.3±4.1 0.25±0.00 35.2±0.1
LP-DOTAP/POPC 115.5±2.0 0.26±0.01 46.0±1.6 233.1±3.0 0.28±0.01 31.3±0.6
a

In water.

b

Charge ratio (+:-) of cationic lipid to siRNA phosphate, 4:1. Data are presented as the mean ± SD (n=3). DOTAP, 1,2-dioleoyl-3-trimethylammonium-propane methyl sulfate salt; PDI, polydispersity index; DSPC, 1,2-distearoyl-sn-glycero-3-phosphocholine; DPPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; DMPC, 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; DSPE, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine; DMPE, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine; POPE, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine; LP, liposome.