TABLE 2.
Quantification of SRB in Smeerenburgfjorden sediments by FISH and rRNA slot blot hybridization
| Depth (cm) | Absolute cell no. (109) ml−1 | Bacteria (probe EUB338): % DAPI | Concn of prokaryotic RNA (EUB338 + Arch915) (ng ml−1) |
Desulfovibrio spp. (DSV698)
|
Desulforhopalus spp. (DSR651)
|
||||
|---|---|---|---|---|---|---|---|---|---|
| % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | ||||
| 0.25 | 2.9/2.1a | 57.9/72.9 | 9,865/8,977 | 0.9/0.8 | 220/338 | 2.2/3.8 | 1.1/0.9 | 257/207 | 2.6/2.3 |
| 0.75 | 3.5/3.3 | 55.9/73.6 | 10,036/13,846 | 1.1/1.6 | 264/521 | 2.6/3.8 | 1.5/1.1 | 146/547 | 1.5/4.0 |
| 1.25 | 3.1/3.4 | 56.1/65.4 | 7,321/10,597 | 0/0.8 | 178/349 | 2.4/3.3 | 2.1/1.6 | 284/571 | 3.9/5.4 |
| 1.75 | 4.2/4.2 | 53.1/57.7 | 6,879/8,325 | 0.6/0 | 152/266 | 2.2/3.2 | 2.3/1.3 | 195/202 | 2.8/2.4 |
| 2.25 | 4.1/3.5 | 57.5/48.4 | 7,161/9,605 | 0.4/0 | 171/329 | 2.4/3.4 | 2.2/1.5 | 91/502 | 1.3/5.2 |
| 2.75 | 3.7/3.3 | 47.9/51.2 | 5,037/5,866 | 0 | 119/176 | 2.4/3.0 | 3.2/2.3 | 94/96 | 1.9/1.6 |
| 3.25 | 2.7/3.2 | 43.7/46.6 | 4,724/6,417 | 0 | 118/177 | 2.5/2.8 | 3.0/2.4 | 138/129 | 3.0/2.0 |
| 3.75 | 3.1/3.7 | 42.8/50.0 | 5,212/6,671 | 0 | 128/206 | 2.5/3.1 | 2.9/2.3 | 145/151 | 2.8/2.3 |
| 4.25 | 3.5/3.4 | 43.5/47.3 | 3,613/5,070 | 0 | 94/133 | 2.6/2.6 | 2.7/1.7 | 86/86 | 2.4/1.7 |
| 4.75 | 3.4/3.6 | 28.4/42.2 | 3,605/4,427 | 0 | 107/113 | 3.0/2.5 | 2.7/1.7 | 132/75 | 3.7/1.7 |
| 5.5 | 3.5/4.6 | 26.0/36.0 | 2,616/4,046 | 0 | 77/115 | 3.0/2.9 | 1.8/1.8 | 48/120 | 1.8/3.0 |
| 6.5 | 3.7/4.7 | 24.1/27.4 | 2,984/4,494 | 0 | 90/117 | 3.0/2.6 | 1.9/1.3 | 67/91 | 2.2/2.0 |
| 7.5 | 4.7/3.9 | 23.6/23.6 | 2,662/3,520 | 0 | 77/98 | 2.9/2.8 | 1.5/0.8 | 65/69 | 2.5/2.0 |
| 8.5 | 2.9/3.1 | 28.9/22.6 | 2,869/3,246 | 0 | 82/92 | 2.9/2.8 | 1.5/1.3 | 76/78 | 2.6/2.4 |
| 9.5 | 3.7/3.9 | 22/15.7 | 2,308/2,898 | 0 | 64/83 | 2.8/2.8 | 1.6/1.2 | 89/58 | 3.9/2.0 |
|
Desulfobulbus spp. (660)
|
Desulfotalea spp. (Sval430)
|
Desulfobacter spp. (DSB985)
|
Desulfobacterium spp. (221)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) |
| 0 | 19/31 | 0.2/0.4 | 0 | 113/35 | 1.2/0.4 | 0 | 45/57 | 0.5/0.7 | 0 | 0 |
| 0 | 19/38 | 0.2/0.3 | 0 | 229/71 | 2.3/0.5 | 0 | 67/67 | 0.7/0.5 | 0 | 0 |
| 0 | 22/30 | 0.3/0.3 | 0/0.8 | 229/59 | 3.1/0.6 | 0 | 76/71 | 1.0/0.7 | 0 | 0 |
| 0 | 19/31 | 0.3/0.4 | 1.3/1.2 | 180/25 | 2.6/0.3 | 0 | 28/128 | 0.4/1.5 | 0 | 0 |
| 0 | 20/22 | 0.3/0.2 | 1.1/1.0 | 256/32 | 3.6/0.3 | 0 | 49/60 | 0.7/0.6 | 0.5/0 | 0 |
| 0 | 15/19 | 0.3/0.3 | 0.8/1.0 | 167/46 | 3.3/0.8 | 0 | 15/45 | 0.3/0.8 | 2.2/0 | 0 |
| 0 | 9/23 | 0.2/0.4 | 0.7/1.4 | 173/71 | 3.7/1.1 | 0 | 5/0 | 0.1/0 | 1.7/0.6 | 0 |
| 0 | 10/28 | 0.2/0.4 | 1.2/1.3 | 293/230 | 5.6/3.4 | 0 | 0 | 0 | 0.7/0 | 0 |
| 0 | 7/19 | 0.2/0.4 | 1.0/1.8 | 114/41 | 3.2/0.8 | 0 | 0 | 0 | 0 | 0 |
| 0 | 7/15 | 0.2/0.4 | 0.6/1.6 | 114/31 | 3.2/0.7 | 0 | 0 | 0 | 0 | 0 |
| 0 | 4/13 | 0.2/0.3 | 1.4/1.5 | 64/35 | 2.5/0.9 | 0 | 0 | 0 | 0 | 0 |
| 0 | 6/10 | 0.2/0.2 | 0.4/1.4 | 61/38 | 2.0/0.9 | 0 | 0 | 0 | 0 | 0 |
| 0 | 5/7 | 0.2/0.2 | 0.6/1.5 | 42/39 | 1.6/1.1 | 0 | 0 | 0 | 0 | 0 |
| 0 | 5/8 | 0.2/0.2 | 1.3/1.0 | 32/29 | 1.1/0.9 | 0 | 0 | 0 | 0 | 0 |
| 0 | 5/6 | 0.2/0.2 | 0.5/0 | 25/21 | 1.1/0.7 | 0 | 0 | 0 | 0 | 0 |
|
Desulfosarcina spp. and Desulfococcus sp. (probe DSS658)
|
Sum for SRB detected
|
DSS clones (probe DSS225): % DAPI | Clones Sva0081 and Sva0863 (probe cl81-644): % DAPI |
Desulfuromonas spp. (probe DRM432)
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | % DAPI | RNA concn (ng ml−1) | % of prokaryotic RNA | ||
| 1.7/1.8 | 465/555 | 4.7/6.2 | 3.7/3.5 | 1,120/1,224 | 11.4/13.6 | 1.2/0.7 | 0.7/0.7 | 0.7/0.7 | 201/214 | 2.0/2.4 |
| 3.1/3.1 | 562/1,180 | 5.6/8.5 | 5.7/5.7 | 1,286/2,423 | 12.8/17.5 | 2.3/1.8 | 1.0/1.4 | 1.5/1.1 | 182/490 | 1.8/3.5 |
| 4.7/4.5 | 648/1,311 | 8.9/12.4 | 6.8/7.7 | 1,437/2,391 | 19.6/22.6 | 3.3/2.8 | 1.6/1.8 | 1.9/1.5 | 239/535 | 3.3/5.1 |
| 6.5/6.7 | 906/848 | 13.2/10.2 | 10.6/9.3 | 1,481/1,500 | 21.5/18.0 | 4.0/3.9 | 1.6/2.6 | 1.4/1.5 | 283/349 | 4.1/4.2 |
| 8.9/9.5 | 595/1,066 | 8.3/11.1 | 13.2/12.1 | 1,181/2,011 | 15.5/20.9 | 6.3/5.6 | 2.4/2.7 | 1.9/2.2 | 150/434 | 2.1/4.5 |
| 9.3/11.0 | 743/498 | 14.8/8.5 | 15.6/14.3 | 1,152/880 | 22.9/15.0 | 6.6/7.0 | 2.6/2.9 | 1.7/1.7 | 212/236 | 4.2/4.0 |
| 10.0/11.7 | 727/522 | 15.4/8.1 | 15.4/16.1 | 1,169/922 | 24.8/14.4 | 7.4/6.1 | 2.3/1.7 | 1.3/1.4 | 235/247 | 5.0/3.8 |
| 8.8/7.4 | 929/836 | 17.8/12.5 | 13.6/10.9 | 1,504/1,449 | 28.9/21.7 | 5.4/4.6 | 1.2/2.3 | 1.2/0.7 | 291/351 | 5.6/5.3 |
| 8.8/8.1 | 608/450 | 16.8/8.9 | 12.5/11.6 | 910/729 | 25.2/14.4 | 6.4/3.9 | 2.5/2.7 | 0.8/0.5 | 205/149 | 5.7/2.9 |
| 6.0/6.2 | 559/590 | 15.5/13.3 | 9.2/9.5 | 918/823 | 25.5/18.6 | 3.8/4.1 | 1.6/0.6 | 1.3/1.0 | 229/191 | 6.4/4.3 |
| 6.6/5.2 | 305/651 | 11.7/16.1 | 9.8/8.5 | 499/933 | 19.1/23.1 | 3.7/3.6 | 0.9/3.1 | 1.3/1.0 | 104/246 | 4.0/6.1 |
| 7.1/6.5 | 378/725 | 12.7/16.1 | 9.4/9.1 | 601/981 | 20.1/21.8 | 5.2/3.4 | 1.9/1.8 | 0.9/1.0 | 86/191 | 2.9/4.3 |
| 5.2/5.6 | 378/519 | 14.2/14.7 | 7.3/7.9 | 567/732 | 21.3/20.8 | 3.3/2.8 | 1.8/1.3 | 1.1/0.7 | 68/129 | 2.6/3.7 |
| 4.4/4.2 | 442/492 | 15.4/15.2 | 7.3/6.4 | 637/698 | 22.2/21.5 | 2.4/2.6 | 0.5/0.7 | 0.8/0.6 | 91/126 | 3.2/3.9 |
| 3.0/3.6 | 393/350 | 17.0/12.1 | 5.1/4.7 | 575/519 | 24.9/17.9 | 1.9/1.0 | 1.0/1.5 | 0.5/0.2 | 93/136 | 4.0/4.7 |
a
Core A value/core B value.