KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| HRP-conjugated anti-Flag antibody | Sigma | Cat# A8592; RRID:AB_439702 |
| Mouse anti Actin antibody | Santa Cruz | Cat# SC-47778; RRID:AB_626632 |
| Anti-mouse IgG, HRP linked antibody | Cell Signaling | Cat#7076S; RRID:AB_330924 |
| Bacterial and virus strains | ||
| NEB 5-alpha | NEB | Cat#C2987H |
| Chemicals, peptides, and recombinant proteins | ||
| Antimycin A | Abcam | Cat#141904 |
| Yeast nitrogen base without amino acid and ammonium sulfate | BD | Cat#233520 |
| Ammonium Sulfate | VWR | Cat# BDH9216-2.5KG |
| Glucose | Sigma | Cat# G8270-10KG |
| Ethanol | VWR | Cat# 71001-866 |
| Potassium Acetate | VWR | Cat# BHD9254-500G |
| Adenine Sulfate | Amersco | Cat# 0607-50G |
| Alanine | VWR | Cat# AAJ60279-18 |
| Arginine | Alfa Aesar | Cat# A14730 |
| Asparagine | VWR | Cat# AAJ62869-22 |
| Aspartic acid | Chem Impex | Cat# 00027 |
| Cysteine | VWR | Cat# AAA10389-22 |
| Glutamine | VWR | Cat# AAJ60573-22 |
| Glutamic acid | Sigma | Cat# G8415 |
| Glycine | VWR | Cat# 0167-5KG |
| Inositol | VWR | Cat# A13586-22 |
| Isoleucine | VWR | Cat# AAA13699-18 |
| Leucine | VWR | Cat# AAJ62824-22 |
| Lysine | VWR | Cat# AAJ62225-14 |
| Methionine | Chem Impex | Cat# 00221-100G |
| para-Aminobenzoic acid | Sigma | Cat# A9878 |
| Phenylalanine | Santa Cruz | Cat# sc-394058 |
| Proline | VWR | Cat# AAA10199-22 |
| Serine | VWR | Cat# AAA11179-14 |
| Threonine | Alfa Aesar | Cat# A16851 |
| Tryptophan | VWR | Cat# AAA10230-14 |
| Tyrosine | Alfa Aesar | Cat# A11141 |
| Valine | Sigma | Cat# 94619 |
| Histidine | Sigma | Cat# H6034 |
| Uracil | VWR | Cat# AAA15570-18 |
| Agar | Millipore | Cat# 12177 |
| Trisbase | Santa Cruz | Cat# sc-3715C |
| Sodium Chloride | VWR | Cat# BDH9286-2.5KG |
| NP40 | VWR | Cat# 97064-730 |
| 3XFlag peptide | Sigma | Cat# F4799-25MG |
| Triethylammonium bicarbonate | Sigma | Cat# T7408 |
| Urea | Fisher Scientific | Cat# BP169-212 |
| Thiourea | Fisher Scientific | Cat# AC424540010 |
| Sodium lauryl sulfate | Fisher Scientific | Cat# S529-500 |
| Dithiothreitol | Fisher Scientific | Cat# BP172-5 |
| Iodoacetamide | Fisher Scientific | Cat# 122271000 |
| Phosphoric acid | Fisher Scientific | Cat# A260-500 |
| AspN | Promega | Cat# VA1160 |
| Formic acid | Fisher Scientific | Cat# A117-50 |
| Acetonitrile | Fisher Scientific | Cat# A955-4 |
| Lysine-13C6,15N2 hydrochloride | Sigma | Cat# 608041 |
| Arginie-13C6,15N4 hydrochloride | Sigma | Cat# 608033 |
| Sodium hydroxide | VWR | Cat# 97064-486 |
| 2-Mercaptoethanol | VWR | Cat# 97064-878 |
| Trichloroacetic acid | Sigma | Cat# T6399-100G |
| Sodium phosphate dibasic | Sigma | Cat# S9763 |
| Hydrochloric acid | VWR | Cat# 470301-260 |
| Potassium Chloride | Sigma | Cat# P9541 |
| Magnesium Chloride | VWR | Cat# BDH9244-500G |
| Critical commercial assays | ||
| Extracellular O2 Consumption Assay | Abcam | Cat#197243 |
| Deposited data | ||
| Proteomics Dataset | ProteomeXchange | PXD032321 |
| Experimental models: Organisms/strains | ||
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Horizon Discovery | YSC1048 |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX | Horizon Discovery | YSC6273-201937826 |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Adh2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX adh2Δ0∷Adh2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Adh2(1–4) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Adh2(1–9) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Adh2(1–14) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷Adh2(1–19) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷ Adh2(1–24) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 adh2Δ0∷ Adh2(1–31) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX adh2Δ0∷Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX adh2Δ0∷Adh2(1–4) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMXadh2Δ0∷Adh2(1–9) Rluc This paper 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMXadh2Δ0∷Adh2(1–14) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMXadh2Δ0∷Adh2(1–19) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX adh2Δ0∷ Adh2(1–24) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX adh2Δ0∷ Adh2(1–31) Rluc 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 atp2Δ0∷Atp2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX atp2Δ0∷Atp2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ssa1Δ0∷Ssa1 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMXssa1Δ0∷Ssa1 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Lsc2Δ0∷Lsc2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX Lsc2Δ0∷Lsc2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Sod2Δ0∷Sod2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX Sod2Δ0∷Sod2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Trr2Δ0∷Trr2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX Trr2Δ0∷Trr2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Rki1Δ0∷Rki1 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX RkiΔ0∷Rki1 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Tdh2Δ0∷Tdh21 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX Tdh2Δ0∷Tdh2 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Tom70Δ0∷Tom70 3XFlag∷His3 | This paper | N/A |
| MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lia1Δ0∷KANMX Tom70Δ0∷Tom70 3XFlag∷His3 | This paper | N/A |
| Oligonucleotides | ||
| ADH2-RT-F: GCCGCTCACATTCCTCAAGGTAC |
IDT | N/A |
| ADH2-RT-R: GCCCAGTGGCCTGCTCTC |
IDT | N/A |
| SOD2-RT-F: TCAAGTTCCATGGCGGTGG |
IDT | N/A |
| SOD2-RT-R: GCCAAAGCGCCGGTGG |
IDT | N/A |
| ACT1-RT-F: TGGCCGGTAGAGATTTGACTGAC |
IDT | N/A |
| ACT1-RT-R: GTTCGAAGTCCAAGGCGACG |
IDT | N/A |
| Other | ||
| Anti-Flag® M2 affinity gel | Sigma | Cat# A2220-25ML |
| Micro Bio-Spin® column | Bio-rad | Cat# 732-6221 |
| S-Trap micro spin column | PROTIFI | Cat# C02-micro-80 |
| Acclaim PepMap C18 RP trapping column | Thermo Scientific | Cat# 164564-CMD |
| Acclaim PepMap C18 RSLC Nano column | Thermo Scientific | Cat# 164,941 |
| PVDF membrane | Biorad | Cat# 1620177 |
| 400 micron Acid Washed Zirconium Beads | Ops Diagnostics | Cat# BAWZ 400-250-35 |