KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-mouse CD45 BUV395 (clone 30-F11) | BD Biosciences | Cat# 564279; RRID: AB_2651134 |
| anti-mouse CD45 Pacific Blue (clone 30-F11) | Biolegend | Cat# 103126; RRID: AB_493535 |
| anti-mouse CD4 BV605 (clone RM4-5) | Biolegend | Cat# 100548; RRID: AB_11125962 |
| anti-mouse CD4 Pacific Blue (clone RM4-5) | Biolegend | Cat# 100531; RRID: AB_493375 |
| anti-mouse CD4 PE (clone RM4-5) | Biolegend | Cat# 100512; RRID: AB_312715 |
| anti-mouse CD4 Pacific Blue (clone RM4-4) | Biolegend | Cat# 116007; RRID: AB_11147758 |
| anti-mouse CD69 PerCP-Cy5.5 (clone H1.2F3) | BD Biosciences | Cat# 551113; RRID: AB_394051 |
| anti-mouse CD90.2, Thy1.2 PE (clone 53-2.1) | BD Biosciences | Cat# 553006; RRID: AB_394545 |
| anti-mouse CD64 BV605 (clone X54-5/7.1) | Biolegend | Cat# 139323; RRID: AB_2629778 |
| anti-mouse CD19 BV786 (Clone 1D3) | Biosciences | Cat# 563333; RRID: AB_2738141 |
| anti-mouse F4/80 PE-Cy5 (Clone BM8) | Invitrogen | Cat# 25-4801-82; RRID: AB_469653 |
| anti-mouse CD88 APC (Clone 20/70) | Biolegend | Cat# 135808; RRID: AB_10899415 |
| anti-mouse MHC-II APC-ef780 (Clone M5/114.14.2) | eBioscience | Cat# 47-5321-82; RRID: AB_1548783 |
| anti-mouse IFNg APC (clone XMG1.2) | Ebioscience | Cat# 17-7311-82; RRID: AB_469504 |
| anti-mouse CD11c FITC (clone N418) | Ebioscience | Cat# 11-0114-85; RRID: AB_464941 |
| anti-mouse CD11c BV711 (clone N418) | Biolegend | Cat# 117349; RRID: AB_2563905 |
| anti-mouse CD26 PE-Cy7 (clone H194-112) | Biolegend | Cat# 137810; RRID: AB_2564312 |
| anti-mouse XCR1 BV650 (clone ZET) | Biolegend | Cat# 148220; RRID: AB_2566410 |
| anti-mouse Ly6G FITC (clone 1A8) | Biolegend | Cat# 127606; RRID: AB_1236494 |
| anti-mouse CD3e Biotin (clone 145-2C11) | eBioscience | Cat# 13-0031-82; RRID: AB_466319 |
| anti-mouse CD31 AF647 (clone MEC13.3) | Biolegend | Cat# 102416: RRID: AB_493410 |
| anti-mouse MHC-II AF647 (clone M5/114) | Biolegend | Cat# 107618, RRID: AB_493525 |
| anti-mouse LYVE-1 AF647 (clone 223322) | R&D Systems | Cat# FAB2125R |
| Ghost Dye Violet 510 | Tonbo biosciences | Cat# 13-0870 |
| anti-mouse IL-4 (clone 11B11), purified | ATCC | Cat# HB-188; RRID: CVCL_9139 |
| anti-mouse IFNg (clone XMG1.2), purified | N/A | N/A |
| anti-mouse CD4 (clone GK1.5) | BioXcell | Cat# BE0003-1 |
| anti-mouse MHC-II (clone M5/114) | BioXcell | Cat# BE0108 |
| CD16/CD32 Monoclonal antibody (Fc block clone 2.4G2) | N/A | N/A |
| JIJ monoclonal antibody (anti Thy1.2) | ATCC | Cat# TIB-184; RRID: CVCL_9186 |
| Chemicals, peptides, and recombinant proteins | ||
| CellTracker Orange Deep Red Dye | Invitrogen | Cat# C34565 |
| CFSE (5-(and-6)-Carboxyfluroescein Diacetate, Succinimidly Ester) | Invitrogen (Molecular Probes) | Cat# C1157 |
| Complete Freund’s Adjuvant (CFA) | Sigma-Aldrich | Cat# F5881 |
| OVA peptide (Structure: ISQAVHAAHAEINEAGR-OH) | Biopeptide Co., Inc. | Custom peptide |
| OVA protein | Sigma-Aldrich | cat# A5503 |
| KLH protein | Sigma-Aldrich | Cat#H8283 |
| Human rIL-2 | NIH AIDS Reagent Program | Cat# 11697 |
| Recombinant Murine IL-4 | Peprotech | Cat# 214-14 |
| Recombinant Murine IL-12 p70 | Peprotech | Cat# 210-12 |
| Guinea Pig Complement, standard | Cedarlane | Cat# CL5000 |
| Fixation/Permeablization Solution Kit with BD GolgiPlug | BD Biosciences | Cat# 555028 |
| Fluriso (Isoflurane, USP) | VET One | Cat# 502017 |
| Liberase DL Research grade | Sigma Aldrich | Cat# 5466202001 |
| RPMI 1640 | Lonza BioWhittaker | Cat# 12-115F |
| HBSS | Corning Cellgro | Cat# 21-021-CV |
| PBS | Corning Cellgro | Cat# 21-040-CV |
| Fetal Bovine Serum-Premium | Atlanta Biologicals | Cat# S11150 |
| EDTA | Corning Cellgro | Cat# 46-034-CI |
| Streptavidin BV510 | Biolegend | Cat# 405233 |
| Brefeldin A | Invitrogen | Cat# B6542 |
| AccuCheck Counting Beads | Thermo Fisher Scientific | Cat# PCB100 |
| Peanut agglutinin (PNA) | Sigma Aldrich | Cat# L2507 |
| DNase I | Sigma Aldrich | Cat# DN25 |
| Critical commercial assays | ||
| Milliplex MAP Kit, Mouse Cytokine/Chemokine Magnetic Bead Panel | Millipore | Cat# MCYTMAG70PMX32BK |
| Naive CD4+ T cell isolation kit, mouse | Miltenyi | Cat# 130-104-453 |
| Experimental models: Organisms/strains | ||
| Mouse: REX3 mouse | Andrew Luster (MGH) | N/A |
| Mouse: B6.PL674 Thy1a/CyJ (B6 Thy1.1) | The Jackson Laboratory | Stock No: 000406 |
| Mouse: B6(Cg)-Tyrc-2J/J (Albino B6) | The Jackson Laboratory | Stock No: 000058 |
| Mouse: OTII TCR Transgenic B6 | The Jackson Laboratory | Stock No: 004194 |
| Mouse: B6.129P2-Cxcr3tm1Dgen/J (B6 CXCR3 KO) | The Jackson Laboratory | Stock No: 005796 |
| Mouse: Kaede B6 | M. Tomura, Kyoto University, RIKEN | Stock No: RBRC05737 |
| Parasite: Leishmania Major strain WHOM/IR/-/173 | R. Locksley (UCSF) | N/A |
| Software and algorithms | ||
| Imaris | Bitplane | RRID: SCR_007370 |
| Flow Jo | Tree Star | RRID: SCR_008520 |
| Python | Python Software Foundation | RRID: SCR_008394 |
| Prism | Graph Pad | RRID: SCR_002798 |
| Other | ||
| Isoflurane vaporizer-ventilation machine | Lei Medical | Model# M3000R |
| LS Columns | Miltenyi | Cat# 130-042-401 |
| Objective heater | Bioptechs | SKU: 150819-19 |
| Objective controller | Bioptechs | SKU: 150803 |
| Olympus FVMPE-RS | Olympus | N/A |
| Olympus FV-MRVGR/XR, filter set | Olympus | N/A |
| Olympus XLPlanN 25x objective | Olympus | Product# N5165100 |
| BD LSR Fortesa | BD Biosciences | N/A |
| BioPlex 200 reader | BioRad | N/A |