. Author manuscript; available in PMC: 2023 Mar 6.

Published in final edited form as: Rapid Commun Mass Spectrom. 2021 Dec 23;38(Suppl 1):e9189. doi: 10.1002/rcm.9189

Table 12.

Aliquoting and Diluting

Processing time	Step	Comments / Tips
30–45 minutes	Remove samples from freezer. Once thawed, aliquot volume containing 50 μg of sample into separate Eppendorf Tubes based off measured protein concentrations. This is important to normalize sample protein for the purpose of performing relative quantification of specific proteins detected in nanoLC-MS/MS analysis.	With large numbers of samples, it is very important to keep track of labelling. Samples will change containers a few times in this protocol.
30–45 minutes	Bring samples up to 200 μL final volume with buffer containing 50 mM ABC and 1% (w/v) SDC to solubilize protein. Vortex.