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Elution of Tryptic Peptides
| Processing time | Step | Comments / Tips |
|---|---|---|
| 45–60 minutes | Centrifuge filtration units for 15 minutes at 12,000 × g to elute peptides. | To maximize tryptic peptide recovery, increase the centrifugation speed to 12,500 × g or 13,000 × g if need be to pass all the digest solution through the filter. |
| Add 15.8 μL 50 mM ABC to elute any remaining peptides on filter. Centrifuge for 15 min at 12,000 × g. |
