Table 2.

Lysis Buffer, 50 mM ABC and 1% SDC (w/v) with Protease Inhibitor, 500 mL

Processing time	Step	Comments / Tips
15–20 minutes	Measure out 2.0 ± 0.1 g ABC and add to glass solvent bottle.	If necessary, this step can be scaled down to preserve reagents. The following should be considered when scaling:  □ 10 mL per sample to submerge sample tissue (more or less, depending on amount of sample)  □ Volume needed as a blank for protein quantitation method  □ Volume need to rinse sonicator probe (∼ 3 mL per sample to prepare 3 fresh tubes for rinsing)
	Measure out 5.0 ± 0.1 g SDC and add to same glass solvent bottle.
	Add 500 mL of water to glass solvent bottle. Shake well until reagents are fully dissolved.
	Add 10 (one for every 50 mL buffer) Roche Protease Inhibitor Tablets. Shake until dissolved.
		Note that lysis buffer should always be kept on ice.