Table 21.
nanoLC Parameters
| Sample pickup and Loading | |||
| Sample Pickup | |||
| Volume | 2 | μL | |
| Flow | 10 | μL/min | |
| Sample Loading | |||
| Volume | 6 | μL | |
| Flow | 1 | μL/min | |
| Max. Pressure | 700 | Bar | |
| Solvents | A: water/acetonitrile + formic acid (98/2 + 0.1 % by volume) | B: water/acetonitrile + formic acid (80/20 + 0.1 % by volume) | |
| Gradient | |||
| Time | Duration | Flow (nL/min) | %B |
| 0:00 | 0:00 | 300 | 5 |
| 2:00 | 2:00 | 300 | 5 |
| 107:00 | 105:00 | 300 | 25 |
| 122:00 | 15:00 | 300 | 40 |
| 123:00 | 1:00 | 300 | 95 |
| 124:00 | 1:00 | 300 | 95 |
| 140:00 | 16:00 | 300 | 95 |
| Pre-column and Analytical Column | |||
| Pre-column Equilibration | |||
| Volume | 3 | μL | |
| Flow | 3.00 | μL/min | |
| Max. Pressure | 700 | Bar | |
| Analytical Column Equilibration | |||
| Volume | 6 | μL | |
| Flow | - | μL/min | |
| Max. Pressure | 700 | Bar | |
*
Custom wash used for Autosampler and column heating controlled externally on Easy-Spray Ion Source. Column is kept at 45 °C throughout analysis.