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Reducing Agent, Dithiothreitol (500 mM)
| Processing time | Step | Comments / Tips |
|---|---|---|
| 15–30 minutes | Weigh out 77.1 ± 0.1 mg of dithiothreitol into an Eppendorf tube. | Prepared DTT solution can be stored at −20°C for at least 1 month. |
| Reconstitute in 1 mL of water. | ||
| Vortex. | ||
| Aliquot solution into Eppendorf tubes in 20 μL volumes and store at −20°C until ready for use. |
