Table 4.

Alkylating Agent, Iodoacetamide (500 mM)

Processing time	Step	Comments / Tips
15–30 minutes	Prepare in as little light as possible. Compound is sensitive to light.	Prepared IAA solution can be stored at −20°C for at least 1 month.
	Weigh out 305 ± 0.1 mg of iodoacetamide into a 15 mL Falcon tube.
	Reconstitute in 3.3 mL of water.
	Vortex.
	Aliquot into Eppendorf tubes (volumes appropriate for a 6 μL aliquot per sample, between 30 μL and 150 μL for example) and store at −20°C in the dark until ready for use.