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50 mM ABC only
| Processing time | Step | Comments / Tips |
|---|---|---|
| 15–30 minutes | Measure out 79.1 ± 0.1 mg ABC into a 50 mL Falcon Tube. | The pH of ABC buffers in this protocol should be about 7.8. |
| Add approximately 5 mL water and vortex until fully dissolved. | ||
| Add water until solution reaches the 20 mL mark on the Falcon Tube. |
