Table 7.

50 mM ABC and 8M Urea

Processing time	Step	Comments / Tips
15–20 minutes	Measure out 79.1 ± 0.1 mg ABC into 50 mL Falcon Tube.	This will seem like a large volume of urea and will take a lot of time to fully dissolve. Add water carefully and slowly to avoid adding over the 20 mL point. Warming in your hands will help with dissolution. Do not heat the solution.
	Measure out 9.8 ± 0.1 g urea into same Falcon Tube.
	Add 10 mL water and vortex until mostly dissolved.
	Bring up to 15 mL mark with water. Vortex for a few minutes until mostly dissolved.
	Add water until solution reaches the 20 mL mark on the Falcon Tube.