Table 8.

Rinsing Blood from Samples

Processing time	Step	Comments / Tips
2–3 hours	Prepare rinsing solution fresh on day of use (see above, Table 1). Solution should always be scaled based on the number of samples and kept on ice after preparation.	This can be done on a separate day. Depending on how many samples are being processed or how many rinses are required to remove as much visible blood as possible, this step may require longer net processing time.
	Add 5 mL protease inhibitor solution to each sample. Samples should be kept on ice throughout entire washing procedure.
	Shake samples on BellyDancer shaker at the highest speed possible while on ice for 5 minutes.
	Replace inhibitor solution with fresh 5 mL, and repeat process five times for a total of 25 minutes of shaking (or more as needed, see tip).