Table 9.

Tissue Homogenization

Processing time	Step	Comments / Tips
3–4 hours	Prepare lysis buffer (see above, Table 2). Lysis buffer should be kept on ice at all times.	This step should take place under a hood. This can be done on a separate day. Depending on how many samples are being processed, this step may require longer net processing time.
	Add 10 mL of cold lysis buffer to each tissue container. Tissue should be submerged in buffer. Tissues should be kept on ice.
	Using the Omni handheld homogenizer under a hood, homogenize each sample individually on ice for 4–5 minutes until sample appears homogeneous in solution. Highest speed (35,000 rpm) is recommended for large amounts of tissue.
	Replace with a fresh homogenizer probe before the next sample is processed. Place the samples on ice after homogenization.