FIG. 2.

Influence of helper oligonucleotides on probe binding at different theoretical temperatures. The lines show the sigmoid melting curves calculated from the respective data points. The values in parentheses are excluded from the fitting procedure. a.u., arbitrary units. The error bars indicate standard deviations calculated from triplicate measurements. (A) E. coli hybridized with Eco603 (solid circles and solid line), Eco603-H621 (open circles and dotted line), and Eco603-H621L (triangles and dashed line). (B) E. coli hybridized with Eco711 (solid circles and solid line), Eco711-H729 (open circles and dotted line), and Eco711-H745 (triangles and dashed line). (C) A. brasilense hybridized with Eco603-H621azo (inverted triangles and dashed line) and Eco603-H621 (open circles and dotted line) and E. coli hybridized with Eco603x-H621L (triangles and dashed-dotted line), Eco603-H621azo (solid circles and solid line), and Eco603-H621azoL (squares and dashed–double-dotted line).