TABLE 1.

Probe and helper sequences and location of target site on E. coli 16S rRNA positions (5)

Probea	Sequence (5′->3′)b	E. coli target position
H48	TCGACTTGCATGTGTTAG	48–65
H66	GCTGCTTCCTGTTACCGT	66–83
Eco84	TCGTCAGCAAAGCAGCAA	84–101
H102	ACTCACCCGTCCGCCAC	102–118
H119	GGCAGTTTCCCAGACATT	119–136
H422	AAAGTACTTTACAACCCG	422–439
H440-2	TCCCTTCCTCCCCGC	440–454
H455	AAAGGTATTAACTTTACTC	455–473
Eco474	CGGGTAACGTCAATGAGC	474–491
H492	TAGCCGGTGCTTCTTCTG	492–509
H567	CTGCGTGCGCTTTACGCC	567–584
Eco585, H585	TCTGACTTAACAAACCGC	585–602
Eco603, H603	GAGCCCGGGGATTTCACA	603–620
Eco603x	GAGCCCGGAGATTTCACA	603–620
Eco621, H621	AGATGCAGTTCCCAGGTT	621–638
H621L	ATCAGATGCAGTTCCCAGGTT	621–641
H621azo	AAATGCAGTTCCCAGGTT	621–638
H621azoL	ATCAAATGCAGTTCCCAGGTT	621–641
Eco639, H639	CTCAAGCTTGCCAGTATC	639–656
H657	CTACCCCCCTCTACGAGA	657–674
Eco711	TCGCCACCGGTATTCCTC	711–728
H729	GTCCAGGGGGCCGCCT	729–744
H745	ACCTGAGCGTCAGTCTTC	745–762

^aEcoXXX, probes labeled with carboxyfluorescein; HXXX, unlabeled helper oligonucleotides. 

^bSequences modified from reference 10. Modifications are shown in boldface.