TABLE 2.

Relative fluorescence intensities of probes and probe-helper combinations

Probe	Relative probe fluorescencea	Increase	Brightness classb
Eco84	8		V
Eco84 + 48	6	0.7	V
Eco84 + 66	45	5.7	III
Eco84 + 102	38	4.8	IV
Eco84 + 119	17	2.1	V
Eco84 + 66 + 102	87	11.1	I
Eco84 + all	70	8.9	II
Eco474	2		VI
Eco474 + 422	2	1.0	VI
Eco474 + 440-2	43	25.1	III
Eco474 + 455	6	3.3	V
Eco474 + 492	34	19.8	IV
Eco474 + 455 + 492	47	27.4	III
Eco474 + all	75	43.2	II
Eco585	5		VI
Eco585 + 567	47	9.3	III
Eco585 + 603	41	8.1	III
Eco585 + 621	13	2.5	V
Eco585 + 639	28	5.5	IV
Eco585 + all	103	20.3	I
Eco603	9		V
Eco603 + 567	19	2.1	V
Eco603 + 585	39	4.1	IV
Eco603 + 621	49	5.2	III
Eco603 + 639	30	3.2	IV
Eco603 + all	78	8.4	II
Eco621	3		VI
Eco621 + 585	17	5.2	V
Eco621 + 603	42	12.6	III
Eco621 + 639	11	3.3	V
Eco621 + 657	8	2.3	V
Eco621 + all	60	17.9	III
Eco639	5		VI
Eco639 + 585	12	2.4	V
Eco639 + 603	15	2.9	V
Eco639 + 621	9	1.8	V
Eco639 + 657	39	7.9	IV
Eco639 + all	70	14.2	II

^aFluorescence intensities are expressed as percentages of that of the brightest probe, Eco1482. 

^bProbes were grouped according to their relative fluorescences into six classes of brightness: class I (100 to 81% relative fluorescence of Eco1482), class II (80 to 61%), class III (60 to 41%), class IV (40 to 21%), class V (20 to 6%), and class VI (5 to 0%).