TABLE 1.
Segregation pattern of Hygromycin B resistance in T1 progenies of rolB‐transgenic T0 plant lines
| Seeds from plant line (T0) | Number of seeds plated on (MS + 25 mg L−1 Hygromycin B) resistant/sensitive to Hygromycin B (HygR/HygS) | Segregation ratio HygR:HygS | χ 2* | |
|---|---|---|---|---|
| Observed | Expected | |||
| Nontransformed | 0/175 | 0:175 | 3:1 | 525 |
| Vector control (EV2) | 93/41 | 2.26:1 | 3:1 | 2.23 |
| rolB transgenic plant line cRolB1 | 163/26 | 6.2:1 | 6:1 | 0.04 |
| rolB transgenic plant line cRolB2 | 121/45 | 2.68:1 | 3:1 | 0.39 |
| rolB transgenic plant line cRolB3 | 65/46 | 1.41:1 | 2:1 | 3.28 |
| rolB transgenic plant line cRolB7 | 66/34 | 1.94:1 | 2:1 | 0.02 |
| rolB transgenic plant line cRolB8 | 60/36 | 1.66:1 | 2:1 | 0.75 |
Note: Resistance and sensitivity of T1 progenies to Hygromycin B evaluated in terms of segregation ratio and tested for goodness of fit to expected ratio using Chi‐Square test. The critical value is 3.84. χ2* = 3.84 (p value threshold <.05).