Figure 3.

Overview of the basic protocol. Briefly, the phagemid containing the antibody sequence is transformed into HB2151 and the cells are cultured with the antibiotics and isopropyl β- d-1-thiogalactopyranoside (IPTG) to induce the protein expression overnight. The cells are collected and lysed with the polymyxin B to release the antibody from the periplasm. The 6x His tagged antibody can be purified by the nickel-charged affinity resin column on AKTA FPLC system. Fractions containing the antibody are subject to dialysis against PBS. Eventually, ELISA is performed to validate the binding of the antibody to the antigen.