Table 1.
Proteins coimmunoprecipitated with RBL10-YFP-HA from chloroplast lysate (n=3).*
| # | Gene Annotation | Accession Number | MW kDa | Net Enrichment | Occurrence |
|---|---|---|---|---|---|
| 1 | PGK1 - phosphoglycerate kinase 1 | AT3G12780 | 50 | 10.483 | 2 |
| 2 | ATPC1 - ATPase, F1 complex, gamma subunit protein | AT4G04640 | 41 | 4.765 | 2 |
| 3 | Transketolase | AT3G60750 | 80 | 4.765 | 2 |
| 4 | LHCB3 - light-harvesting chlorophyll B-binding protein 3 | AT5G54270 | 29 | 4.765 | 2 |
| 5 | PSB27 - photosystem II family protein | AT1G03600 | 19 | 3.7131 | 2 |
| 6 | ATPase, F0 complex, subunit B/B’, bacterial/chloroplast | AT4G32260 | 24 | 2.6612 | 2 |
| 7 | ZKT - protein containing PDZ domain, a K-box domain, and a TPR region | AT1G55480 | 37 | 2.859 | 2 |
| 8 | APX4 - ascorbate peroxidase 4 | AT4G09010 | 38 | 1.8071 | 2 |
| 9 | TOC159 - translocon at the OEM of chloroplasts 159 |
AT4G02510 | 161 | 1.9833 | 2 |
| 10 | CPN60B, LEN1 - chaperonin 60 beta | AT1G55490 | 64 | 1.906 | 2 |
| 11 | EDA9 - D-3-phosphoglycerate dehydrogenase | AT4G34200 | 63 | 1.906 | 2 |
| 12 | SBPASE - sedoheptulose-bisphosphatase | AT3G55800 | 42 | 1.906 | 2 |
| 13 | CLPC1 - CLPC homologue 1 | AT5G50920 | 103 | 1.906 | 2 |
| 14 | ATPB - ATP synthase subunit beta | ATCG00480 | 54 | 0.99167 | 2 |
| 15 | RPS1 - ribosomal protein S1 | AT5G30510 | 45 | 0.99167 | 3 |
| 16 | RPL2.1 - ribosomal protein L2 | ATCG00830 | 30 | 0.99167 | 2 |
| 17 | Pyridine nucleotide-disulphide oxidoreductase family protein | AT1G74470 | 52 | 0.99167 | 2 |
| 18 | Thioredoxin superfamily protein | AT3G11630 | 29 | 0.95299 | 2 |
| 19 | PRK - phosphoribulokinase | AT1G32060 | 44 | 0.95299 | 2 |
| 20 | FTSH1 - FTSH protease 1 | AT1G50250 | 77 | 0.95299 | 2 |
| 21 | ACP4 - acyl carrier protein 4 | AT4G25050 | 15 | 0.95299 | 2 |
| 22 | CPN20 - chaperonin 20 | AT5G20720 | 27 | 0.95299 | 2 |
Proteins that were identified as at least twice as abundant in the anti-HA sample versus control are described here with their gene annotation, their gene ID, predicted molecular mass, the enrichment in the anti-HA sample compared to the control, and the recurrence out of three experimental replicates. Net enrichment = (normalized total peptide spectra in anti-HA sample – normalized total peptide spectra in control sample) / normalized total peptide spectra in control sample.