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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Plant J. 2021 Oct 15;108(5):1332–1345. doi: 10.1111/tpj.15514

Table 1.

Proteins coimmunoprecipitated with RBL10-YFP-HA from chloroplast lysate (n=3).*

# Gene Annotation Accession Number MW kDa Net Enrichment Occurrence
1 PGK1 - phosphoglycerate kinase 1 AT3G12780 50 10.483 2
2 ATPC1 - ATPase, F1 complex, gamma subunit protein AT4G04640 41 4.765 2
3 Transketolase AT3G60750 80 4.765 2
4 LHCB3 - light-harvesting chlorophyll B-binding protein 3 AT5G54270 29 4.765 2
5 PSB27 - photosystem II family protein AT1G03600 19 3.7131 2
6 ATPase, F0 complex, subunit B/B’, bacterial/chloroplast AT4G32260 24 2.6612 2
7 ZKT - protein containing PDZ domain, a K-box domain, and a TPR region AT1G55480 37 2.859 2
8 APX4 - ascorbate peroxidase 4 AT4G09010 38 1.8071 2
9 TOC159 - translocon at the
OEM of chloroplasts 159
AT4G02510 161 1.9833 2
10 CPN60B, LEN1 - chaperonin 60 beta AT1G55490 64 1.906 2
11 EDA9 - D-3-phosphoglycerate dehydrogenase AT4G34200 63 1.906 2
12 SBPASE - sedoheptulose-bisphosphatase AT3G55800 42 1.906 2
13 CLPC1 - CLPC homologue 1 AT5G50920 103 1.906 2
14 ATPB - ATP synthase subunit beta ATCG00480 54 0.99167 2
15 RPS1 - ribosomal protein S1 AT5G30510 45 0.99167 3
16 RPL2.1 - ribosomal protein L2 ATCG00830 30 0.99167 2
17 Pyridine nucleotide-disulphide oxidoreductase family protein AT1G74470 52 0.99167 2
18 Thioredoxin superfamily protein AT3G11630 29 0.95299 2
19 PRK - phosphoribulokinase AT1G32060 44 0.95299 2
20 FTSH1 - FTSH protease 1 AT1G50250 77 0.95299 2
21 ACP4 - acyl carrier protein 4 AT4G25050 15 0.95299 2
22 CPN20 - chaperonin 20 AT5G20720 27 0.95299 2
*

Proteins that were identified as at least twice as abundant in the anti-HA sample versus control are described here with their gene annotation, their gene ID, predicted molecular mass, the enrichment in the anti-HA sample compared to the control, and the recurrence out of three experimental replicates. Net enrichment = (normalized total peptide spectra in anti-HA sample – normalized total peptide spectra in control sample) / normalized total peptide spectra in control sample.