FIGURE 3.

Editing of OsBADH2 gene for generation of aromatic rice using RNP approach. Acclimatized T₀ edited plants for OsBADH2 gene grown under glasshouse conditions (A), Detection of RNP-based editing in the T₀ generation through mutation site based specific primers technique (MSBSP). Encircled lane depicts the mutation (B), A electropherogram showing the result of Sanger sequencing (C), Multiple sequence alignment of putative T₀ plants showing the addition of a nucleotide “A” 4-bp upstream of the PAM site (D), The ORF of OsBADH2 exon seven in PR114 (E), The ORF of Osbadh2 exon seven in the edited plant, 11-4 showing change in the last four amino acid sequences indicating the disruption of protein chain (F).