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. 2022 Jun 22;15:69. doi: 10.1186/s13068-022-02163-5

Fig. 1.

Fig. 1

Analysis of the effect of quenching ROS on cultivated cyanobacterial cell growth by adding ascorbic acid. Cell growth curves of Synechococcus cscB+ (A), H2O2 content (B) and ROS level (C) in the coculture system and axenic culture with additional ascorbic acid. The coculture system were cultivated in coculture medium (named CoBG-11) under a light intensity of approximately 100 μmol photons m−2 s−1 in an illuminating shaking incubator at 150 rpm and 30 °C. CoBG-11 medium was designed based on BG-11 medium and optimized for E. coli growth by supplementing with 150 mM NaCl, 4 mM NH4Cl and 3 g/L 2-[[1,3-dihydroxy-2-(hydroxymethyl) propan-2-yl] amino] ethanesulfonic acid (TES). NH4Cl were used to maintain the cell survival of E. coli, and NaCl was used as a stress inducer for sucrose accumulation in Synechococcus 2973