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. 2022 Jun 23;21:135. doi: 10.1186/s12943-022-01600-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — circEXOC6B binds to RRAGB to interfere with its heterodimer formation. A FISH showed that circEXOC6B was mainly resided in the cytoplasm of CRC cells; Red: circEXOC6B, Blue: nuclei. B RNA pull-down and mass spectrometry (MS) were performed to identify proteins binding to circEXOC6B. The red frame marked RRAGB. C RNA pull-down followed by western blot confirmed the binding of circEXOC6B and RRAGB. D RIP assay indicated that RRAGB could enrich circEXOC6B. E FISH and IF labeled circEXOC6B and RRAGB in CRC cells. F RRAGB expression vectors of different lengths (#1–3) were constructed using pCDNA3.1-Flag vector. G RIP assay showed that the 223–346-aa region of RRAGB was responsible for the interaction with circEXOC6B. H The 223–346-aa region of RRAGB contains the binding site for the heterodimerization of RRAGB with RRAGC/D (235–306-aa, NCBI gene database). I Co-IP demonstrated that the binding of RRAGC and RRAGD to RRAGB decreased with circEXOC6B overexpression