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. 2022 Jun 23;21:135. doi: 10.1186/s12943-022-01600-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — circEXOC6B enhances the sensitivity of CRC cells to 5-FU. A TUNEL showed that knockdown of circEXOC6B expression reduced the 5-FU-induced apoptosis of CRC cells, whereas overexpression of circEXOC6B made CRC cells more sensitive to 5-FU treatment. Scale: 50 μm. B Western blot results showed that knockdown or overexpression of circEXOC6B affected the expression of apoptotic markers in CRC cells after 5-FU treatment. C CCK8 revealed that downregulation of circEXOC6B antagonized the inhibitory role of 5-FU in CRC cell proliferation. In contrast, upregulation of circEXOC6B enhanced the effect of 5-FU on proliferation. D Gross image of subcutaneous tumors. E The tumor volume in the sh-circEXOC6B group showed no difference from the untreated group, whereas the tumor size was significantly decreased in the sh-NC group after 5-FU treatment. F IHC demonstrated that the apoptotic marker cleaved-caspase3 was not increased in the sh-circEXOC6B group but was dramatically elevated in the sh-NC group after 5-FU treatment. Scale: 20 μm. G TUNEL showed that the apoptosis in the sh-circEXOC6B group was unchanged with 5-FU treatment, whereas it was remarkably increased in the sh-NC group after 5-FU treatment. Scale: 50 μm