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. 2022 Jun 23;21:134. doi: 10.1186/s12943-022-01566-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Gilteritinib upregulated the expression of NKG2DLs and FLT3 in MOLM-13 and MV4-11 cell lines. A Flow cytometry analysis showed that the expression of NKG2DLs (MICA/B and ULBP1/3) in MOLM-13 and MV4-11 cells was significantly upregulated with gilteritinib treatment. Histograms show NKG2DL expression on MOLM-13 cells and MV4-11 cells in the absence (grey) and presence (black) of IC25-gilteritinib for 24 h. Inset numbers show the ratio in MFI of treated/nontreated cells. B Western blotting (left) showed that the protein levels of all NKG2DLs in the MOLM-13 cell line were significantly increased with gilteritinib treatment, but in the MV4-11 cell line, only the MICA and ULBP1 levels were upregulated. The diagrams (right) show the relative expression as the grey intensity ratio of the NKG2DL (MICA ~ B, ULBP1 ~ 2) protein band to the internal control (C) Flow cytometry analysis of FLT3 expression on cell lines (MOLM-13 and MV4-11) with or without gilteritinib treatment. The histograms show FLT3 expression in MOLM-13 and MV4-11 cells treated without gilteritinib (0), 24-h IC25 gilteritinib (1), and 24-h IC50 gilteritinib (2). D Western blotting (left) indicated that FLT3 expression in both cell lines was significantly upregulated by gilteritinib pre-treatment. The diagrams (right) show the relative expression as the grey intensity ratio of the FLT3 protein band to the internal control. G, gilteritinib; FITC, fluorescein isothiocyanate; MFI, mean fluorescence intensity. ns, not significant. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001