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. 2022 Jun 9;13:862709. doi: 10.3389/fphar.2022.862709

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Copyright © 2022 He, Wang, Yang, Ma, Qin, Yan, Rong, Xie, Li, Si, Li and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nar inhibited high-glucose-induced VSMC proliferation. (A) CCK-8 assay showed that Nar reduced the viability of high-glucose-induced VSMCs at 24 and 48 h, p < 0.05. (B,C) Flow cytometry showed a lower cell proliferation rate in the Nar group than in the HG group. (D) Immunofluorescent staining of PCNA (magnification: 200×). (E,F) Western blot analysis showed PCNA expression associated with different treatments of VSMCs, ^* p < 0.05 compared with Control group, ^## p < 0.01 compared with HG group.