Figure 2.

Size exclusion chromatography of Aβ40-WT, Aβ42-WT, Aβ40-Dutch, and Aβ40-Iowa. Size exclusion chromatograms are shown for the Aβ peptides incubated at (A–D) ∼25 and (E–H) 37 °C as a function of incubation time. Generally, these peptides elute in two major fractions. The first fraction at ∼8 mL contains protofibrils, while the second fraction at ∼17 mL contains monomers and oligomers. Stable fibrils are excluded from entering the SEC column. The samples were prepared at 4–6 °C at time zero and immediately injected into the SEC column, which was at room temperature.