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FTIR spectra of Aβ40-Dutch and Aβ40-Iowa. FTIR spectra were obtained of the (A and B) Aβ40-Dutch and (C and D) Aβ40-Iowa peptides as a function of incubation time at 25 and 37 °C. For each incubation time point, samples were run in parallel with and without 13C labeling (blue trace) and with 13C-LAGG labeling (red trace) having backbone 13C=O labels at Leu17, Ala21, Gly33, and Gly37. The final spectra were normalized to the intensity of the amide II absorbance band.
