Table 1.
PCR primers used for red panda amdoparvovirus detection and sequencing.
| Purpose and primer | Binding sitea | Amplicon size (nt) | Target ORF | Primer sequence |
|---|---|---|---|---|
|
| ||||
| Screening assay | ||||
| RP Amdo NSF2 | 936–955 | 154 | NS | CGCCAAAACCAACCGACCAA |
| RP Amdo NSR2 | 1069–1089 | AACACGCCCTTAGCTGTGCT | ||
| Full coding sequence | ||||
| Amdo30F | 32–52 | 467 | NS | TGGAATGMGGAAGTKCTRGTG |
| Amdo392R | 479–498 | CCHAMSMAACARTGRATATG | ||
| AmdoNS1.130F | 236–255 | 893 | NS | AAACCAACCAMAGMAACACC |
| AmdoNS1.1058 | 1108–1128 | TCTTCTGGAGTAAAGCAACCA | ||
| Amdo948F | 950–969 | 1052 | NS | GACCAAACTGACTCAGCAAC |
| Amdo1999R | 1981–2001 | GTGAGCTGCCAGAACTCTTGT | ||
| Amdo4F | 1572–1590 | 677 | NS-VP | AAGCYATTACTGGAGGWGG |
| Amdo5R | 2229–2248 | TTGCTGRATAAAYACGTGTC | ||
| Amdo5F | 2094–2111 | 754 | NS-VP | AAGAGACCTCGGCATGAG |
| Amdo2844R | 2826–2847 | CCTGTAACGACGCAGTTAAGTC | ||
| SKAV1F | 2636–2647 | 660 | VP | CCAACAAGTAATGACACCWTGG |
| SKAVR1 | 3308–3324 | TCACCCCAAAAGTGACC | ||
| Amdo8Fb | 3181–3200 | 827 | VP | CCAAAGTACACGTTGCTTGG |
| Amdo4692R | 3985–4009 | CAGTGAGGTTCTCTGTTAGTTTAA | ||
| Amdo4556F | 3906–3927 | 422 | VP | CTGATGTGGACCACAAACCTAG |
| Amdo8R | 4308–4329 | GGTTRTTCACTDTTYATGTAAC | ||
a
All nucleotide positions refer to the previously published genome NC_031751.