Table 2.
Primers and quantitative PCR conditions used for real-time PCR.
| Primer Name 1 | Target Sequence | Sequence (5′–3′) | Conc. 2 (μM) | AT 3 (°C) | Size (bp) | Standard | Reference |
|---|---|---|---|---|---|---|---|
| Bank-lacto-F | All Lactobacillus (23S rRNA) | GCGGTGAAATTCCAAACG | 0.30 | 60 | 216 | Lactobacillus reuteri DSM 20016 | [35] |
| Bank-lacto-R | GGGACCTTAACTGGTGAT | 0.30 | |||||
| E. coli 401 F | All E. coli
(ybbW gene) |
TGATTGGCAAAATCTGGCCG | 0.50 | 65 | 211 | E. coli K12 | [36] |
| E. coli 611 R | GAAATCGCCCAAATCGCCAT | 0.50 | |||||
| 16S_BAC-F (SRV3-1) | All bacteria (16S rRNA) |
CGGYCCAGACTCCTACGG | 0.30 | 65 | 200 | E. coli K12 | [37] |
| 16S_BAC-R (SRV3-2) | TTACCGCGGCTGCTGGCAC | 0.30 |
1 F = forward; R = reverse. 2 Primer concentration. 3 AT = Annealing temperature.