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. Author manuscript; available in PMC: 2022 Jun 23.
Published in final edited form as: Biochim Biophys Acta Proteins Proteom. 2021 Oct 12;1870(1):140732. doi: 10.1016/j.bbapap.2021.140732

Figure 3.

Figure 3.

(A) The electrostatic to covalent cross-linker SBH and the (B) electrostatic to electrostatic cross-linker sulfo-EGS. (C, D) Modified residues represented using MD predicted structures of ubiquitin with protonated and covalent sites labeled in purple and blue respectively. (C) Aqueous 6+ is cross-linked by sulfo-EGS at Pro19 – Arg42 (solid line) and by sulfo-benzoyl HOAt at Lys29 – Pro19 and Arg54 – Lys63 (dashed lines). (D) Under denaturing condition, the pair linked by sulfo-EGS is Lys33 – Arg72 (solid line) and the pairs cross-linked by sulfo-benzoyl HOAt are Lys48 – Lys11 and Arg54 – Lys63 (dashed lines). (C) and (D) Reproduced with permission from ref55. f5552 Copyright 2021 Elsevier. Further permissions related to the material excerpted should be directed to Elsevier.