Figure 4.

Cell death evaluation on salt-treated Baldo and Vialone Nano root apparatus. (A) Relative Root Mortality Ratio (NaCl/C plants) obtained from quantification of Evan’s Blue-stained root extract. (B) Expression of programmed cell death-related gene OsPDCD5 in control and treated plants. Data are presented as the mean standard deviation of at least three biological replicates. In (A) statistical significance determined by two-way ANOVA followed by a Tukey test (p < 0.01). Different letters indicate significant difference. In (B) Student’s t-test was used to evaluate the significant variations between control and treated conditions for each variety. Asterisks indicate significant differences between treatment and control by t-test with p < 0.01. ns, non-significant. The reported values are the means of four independent experiments ± standard deviation.