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. 2022 Jun 11;11(6):1150. doi: 10.3390/antiox11061150

Figure 6.

Figure 6

Lyc W affects inflammation markers in A549 cell line. (A,B) Lyc W significantly increased nuclear translocation of NF-kB expression in comparison to cells treated with medium and in comparison, to cells treated with Lyc G, Lyc T and Lyc S; (A) Three Western blots were semi-quantified by densitometric scanning, normalized and expressed after correction with the density of the bands obtained for housekeeping proteins for nuclear proteins. Data are expressed as arbitrary units. The results are shown as box-plots with medians (lines inside the boxes). Analysis of variance (ANOVA), Fisher’s PLSD, n = three experiments; *, p-value < 0.05. (B) representative Western blot for NF-kB and for the nuclear housekeeping gene Lamin B1; (C,D) Lyc W significantly increased both m-RNA (C) and IL-8 protein expression (D) in comparison to cells treated with medium and in comparison, to cells treated with Lyc G, Lyc T and Lyc S. The results are shown as box-plots with medians (lines inside the boxes). Analysis of variance (ANOVA), Fisher’s PLSD; *, p-value < 0.05. (C) n = three experiments; quantitative real-time PCR of IL-8; (D) n = six experiments, ELISA for IL-8 release.