Figure 4.

Co-ultra PEALut dampens the altered MBP⁺/Olig2⁺ ratio caused by Aβ_1–42. (a) Representative photomicrographs of MBP (red) and Olig2 (green) staining of primary oligodendrocytes obtained using a 20× objective mounted on a fluorescent microscope. Cell nuclei were stained with Hoechst (blue). Scale bar is 40 μm. (b) MBP⁺ cell count. (c) Olig2⁺ cell count. (d) The ratio of MBP⁺ to Olig2⁺ cell counts. Treatments with human fibrillary Aβ_1–42 1 µm in the presence or absence of co-ultra PEALut 3 µm, GW6471 3 µm (or respective vehicles) were added to the astrocytic culture medium. Semi-permeable inserts containing the primary oligodendrocytes were collected 48 h later and processed by immunofluorescence. Data are plotted as mean ± SEM and expressed as percentage variations vs. the vehicle-treated group (CTRL). * p < 0.05; ** p < 0.01 vs. CTRL; ° p < 0.05 vs. Aβ (SNK test).