Table 1.

Western blot conditions.

Primary Antibody	Brand	Dilution	Secondary Antibody	Brand	Dilution
Rabbit α-GFAP	Abcam	1:25,000 5% milk in TBS-T 0.1%	HRP conjugated goat anti-rabbit IgG	Jackson ImmunoResearch	1:10,000 5% milk in TBS-T 0.1%
Rabbit α-GS	Millipore	1:500 5% milk in TBS-T 0.1%	HRP conjugated goat anti-mouse IgG	Jackson ImmunoResearch	1:10,000 5% milk in TBS-T 0.1%
Rabbit α-HMGB1	BioLegend	1:1000 5% milk in TBS-T 0.1%	HRP conjugated goat anti-mouse IgG	Jackson ImmunoResearch	1:10,000 5% milk in TBS-T 0.1%

GFAP: glial fibrillary acidic protein; GS: glutamine synthetase; HMGB1: high mobility group box 1; TBS-T: tris buffered saline tween 20; HRP: horseradish peroxidase.